Biochemical and Enzymatic Analyses to Understand the Accumulation of γ-Aminobutyric Acid in Wheat Grown under Flooding Stress

Flooding induces low-oxygen stress, which reduces plant growth. The activity of the γ-aminobutyric acid (GABA) shunt is crucial for stress adaptation, in it acts by changing cytosolic pH, limiting reactive oxygen species production, regulating nitrogen metabolism, and bypassing steps tricarboxylic cycle. GABA accumulates under osmotic as well flooding stress. To clarify dynamic roles accumulation wheat biochemical enzymatic analyses were performed using a plant-derived smoke solution (PDSS), rescued growth from Alcohol dehydrogenase abundance increased stress; however, same conditions, pyruvic content only following PDSS application. Glutamic but decreased application after 2 days flooding. Glutamate decarboxylase further 1 day PDSS. Succinyl semialdehyde accumulated These results suggest that increases along with increase decrease glutamate succinyl dehydrogenase, respectively. Additionally, at initial stage